Home Page   -   Åditorial Board   -   Instructions to autors   -   Archive   -   Contact   -   Journal's Indexing UA   EN


Last issue

¹ 1 (110), 2024


Support

Edition of the journal is supported partially by the grant of the Fogarty International Center (FIC) of the National Institute of Health and Illinois University, Chicago.

JOURNAL

ISSN 2077-7477 (Print)
ISSN 2077-7485 (Online)

A journal "Dovkillia ta zdorovia" (Environment & Health) publishes the articles on the problems in the field of medical ecology, hygiene, health protection and ecological safety.

Founder of the Journal:
State Institution "O.M. Marzeiev Institute for Hygiene and Medical Ecology of the National Academy of Medical Sciences of Ukraine"

Frequency of publication:
quarterly

Environment & HealthISSN: 2077-7477 eISSN: 2077-7485
No: 4 (89)   -   December, 2018   -   Pages: 4-10
Glutathione-dependent system of antioxidant protection in the animal’s organism under effect of electromagnetic radiation
Tomashevska L.A.1, Kravchun T.Ye.1
1 State Institution "O.M. Marz³eiev Institute for Public Health of the NAMSU"

ÓÄÊ: 613.648.2 : 577.152.1

ABSTRACT:
Objective: We investigated the state of the glutathione-dependent system of antioxidant protection under exposure of electromagnetic radiation in the experiment.

Materials and methods: The investigations were carried out under conditions of 4-month chronic experiment on white non-pure rats which were divided into groups according to the current EMR intensity of 900 MHz (10 μW / cm2, 10 μW / cm2, 10 μW / cm2). We used blood, liver, and brain as a biological material for the investigations.

Results: Strengthening of lipid peroxidation processes in the blood, liver, and brain tissue was established. Those changes were manifested depending on the level of the intensity of EMR and the time of exposure. An increase in the activity of antioxidant glutathione-dependent enzymes – glutathione peroxidase, glutathione reductase, glutathione transferase, as well as a decrease in the level of reduced glutathione, manifested at the increase of intensity (100 and 1000 μW / cm2) of EMR and time (3-4 months of irradiation), were detected. The cooperative antioxidant activity of glutathione and enzymes is insufficient to neutralize the lipid peroxidation process initiated by EMR. Induction of lipid peroxidation and activation of glutathione enzymes indicate a change in pro- and antioxidant balance, a violation of this balance characterizes a degree of stress on the adaptive capabilities of the organism under the influence of EMR.

Conclusions: The identified shifts in the ratio of the intensity of lipid peroxidation and the activity of enzymes of glutathione system as a sensitive biomarker of influence can have prognostic significance for the substantiation of the safe EMR regulations.

KEYWORDS:
electromagnetic radiation, antioxidant activity, glutathione-dependent enzymes
REFERENCES:
1. Serdiuk A.M. and Dumanskyi Yu.D. Elektromahnitna bezpeka – suchasna hihiienichna problema, shliakhy yii vyrishennia [Electromagnetic Safety is a Modern Hygienic Problem, Ways for its Solution]. In : Hihiienichna nauka I praktyka na rubezhi stolit : mater. XVI zizdu hihiienistiv Ukrainy [Hygienic Science and Practice at the Turn of the Century : Materials of the XVI Congress of the Hygienists of Ukraine]. Dnipropetrovsk ; 2004 : 251-254 (in Ukrainian).
2. Dumanskiy V.Yu., Bitkin S.V., Serdiuk Ye.À., and Tomashevskaya L.À. Elektromagnitnoe zagriazneniie okruzhayushchey sredy I zashchita naseleniya ot ego vliyaniya [Electromagnetic Contamination of the Environment and Protection of the Population from its Impact]. In : Hihiiena naselenykh mists [Hygiene of Settlements]. Kyiv ; 2011 ; 58 : 184-189 (in Russian).
3. Maes A. and Verschaeve L. Arch. Toxicol. 2016 ; 90 (10) : 2337-2348. https://doi.org/10.1007/s00204-016-1769-9
4. Directive 2013/35/EU of the European Parliament and of the Council of 26 June 2013 on the Minimum Health and Safety Requirements Regarding the Exposure of Workers to the Risks Arising from Physical Agents (Electromagnetic Fields).
5. Schutz vor elektrischen und magnetischen Feldern der elektrischen Energieversorgung und –anwendung : Empfehlung der Strahlenschutzkommission. Verabschiedet in der 221. Sitzung der Strahlenschutzkommission am 21./22. Februar 2008. 31s .
6. Dimbylow P.J. Radiat Prot Dosimetry. 2006 ; 121(3) : 236-239. https://doi.org/10.1093/rpd/ncl032
7. Berlana T. and Ubeda A. Radiat Prot Dosimetry. 2017 ; 177(4) : 397-406. https://doi.org/10.1093/rpd/ncx058
8. Hlushkov S.I., Kutsenko S.À., Karpysahchenko À.I. and Novikova Ò.Ì. Toksikologicheskiy vestnik. 2003 ; 5 : 6-12 (in Russian).
9. Khudnitskiy S.S. K voprosu vozdeistviya elektromagnitnykh izlucheniy, sozdavaemykh sistemsmi sotovoi sviazi, na polzovatelei I naselenie [To the Issue of the Effect of Electromagnetic Radiation on the Users and Population, Created with the Systems of Cellular Communication]. In : Zdorovie I okruzhaiushchaia sreda : sb. nauch. tr. [Health and Environment : Scientific Work Collection]. Minsk ; 2008 ; 11 : 266-269 (in Russian).
10. Tomashevska L.A., Kravchun Ò.Ye. and Buhaienko Î.Î. Vplyv korotkostrokovoi dii elektromahnitnoho vyprominiuvannia na stsn oksydsntnoi systemy v orhanizmi shchuriv [Impact of Short-Term Effect of Electromagnetic Radiation on the State of Oxidant System in the Rats’ Organism]. In : Hihiiena naselenykh mists [Hygiene of Settlements]. Kyiv ; 2011 ; 57 : 259-264 (in Ukrainian).
11. Zavhorodnii ².V., Bachynskyi R.Î. and Litovchenko Î.L. Dosvid eksperymentalnoho vyvchennia efektiv spoluchenoi dii chimichnykh ta fizychnykh chynnykiv [Experience of the Experimental Study of the Effects of Joint Effect of Chemical and Physical Factors]. In : Aktualni problemy profilaktychnoi medytsyny : zbirnyk [Actual Problems of Prophylactic Medicine]. Lviv ; 2017 ; 1 (14) : 50-58 (in Ukrainian).
12. Daniliva L. À. Spravochnik po laboratornym metodam issledovaniia [Reference Book on the Laboratory Research Methods]. Sankt-Peterburg : Piter ; 2003 : 736 p. (in Russian).
13. Kamyshnikov V.S. Spravochnik kliniko-biokhimicheskim issledovaniiam I laboratornoi diagnostike [Reference Book on Clinical-and-Biochemical Research and Laboratory Diagnostics]. Ìoscow : MEDpressinform ; 2009 : 896 p. (in Russian).
14. OECD. Principles of Good Laboratory Practice.1996.
15. Antomonov M.Yu. Matematicheskaya obrabotka i analiz mediko-biologicheskikh dannykh. 2-e izd. [Mathematical Processing and Analysis of Medico-Biological Data ]. Kiev : Medinform ; 2018 : 579 ð. (in Russian).
16. Sharapova Î.Ì. Ìedychni perspektyvy. 2012 ; XVII (4) : 17-21 (in Ukrainian).
17. Dumanskyi V.Yu., Bitkin S.V., Dumanskyi Yu.D., Nikitina N.G., Serdiuk Ye.À., Polka N.S., Bezverkha À.P., Platonova À.G., Halak S.S., Tomashevska L.À., Zotov S.V., Didyk N.V. and Lemeshko L.P. Hihiienichna otsinka elektromahnitnoho vyprominiuvannia, shcho stvoriuietsia suchasnoiu kompiuternoiu tekhnikoiu [Hygienic Assessment of Electromagnetic Radiation Created with Modern Computer Equipment]. In : Aktualni pytannia zakhystu dovkillia ta zdorovia naselennia Ukrainy (rezultaty naukovykh rozrobok 2014 r.) [Topical Issues of the Protection of Environment and Health of the Population of Ukraine (Results of the Scientific Development in 2014] . Kyiv ; 2015 : 111-160 (in Ukrainian).
Designed by: IPH NAMSU © Environment & Health, 2006-2024. All rights reserved.
Use of text and graphic materials of the site is allowed only upon written permission of the editoral board.