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Edition of the journal is supported partially by the grant of the Fogarty International Center (FIC) of the National Institute of Health and Illinois University, Chicago.

JOURNAL

ISSN 2077-7477 (Print)
ISSN 2077-7485 (Online)

A journal "Dovkillia ta zdorovia" (Environment & Health) publishes the articles on the problems in the field of medical ecology, hygiene, health protection and ecological safety.

Founder of the Journal:
State Institution "O.M. Marzeiev Institute for Hygiene and Medical Ecology of the National Academy of Medical Sciences of Ukraine"

Frequency of publication:
quarterly

Environment & HealthISSN: 2077-7477 eISSN: 2077-7485
No: 4 (75)   -   2015   -   Pages: 13-17
Antimicrobial activity of the composite on the basis of kaolin and silver nanoparticles
Surmasheva O.V.1, Romanenko L.I.1, Korchak G.I.1, Nikonova N.O.1, Gorval A.K.1, Berezovchuk S.M.2, Didikin G.G.2
1 State Institution "O.M. Marzeiev Institute for Public Health of the NAMSU"
2 E.O. Paton Electric Welding Institute of the National Academy of Sciences of Ukraine

: 615.33:678.046.3

ABSTRACT:
The purpose of the study. The study of antimicro-bial activity and physical properties of composite on the basis of kaolin and silver nanoparticles was the purpose of the study.

Materials and methods. The composite on the basis of the preparation of kaolin "Kremnevit" and silver nanoparticles (NPS Ag) obtained by electron-beam evaporation in vacuum with subsequent deposition from the vapour phase has been created. Antimicrobial activity against E. coli was determined by suspension method. As the control was a solution of silver nitrate. Determination of the size, shape and distribution of NPS Ag has been performed by a scanning electron micro-scope of Tescan Vega GP. The stability of aqueous suspensions of the composite was recorded by laser correlation spectrometry in spectrometer "Zeta Sizer-3", the concentration of silver by the method of atomic emission spectroscopy.

The research results and their discussion. The obtained composite had high antimicrobial activity against E. coli. Composite diluted to a concentration of 0,27 cg/g by silver exerted a bactericidal effect on microorganisms at a concentration of (4.5 x 106) CFU/ml for 24 hours, and diluted up to 2.7 mcg/g for 4 hours of contact. Electron microscopy sized NPS Ag for the level of 10-60 nm, NPS Ag had a spherical shape. The most common size of the nanoparticles of kaolin is from 60-140 nm. NPS Ag form a stable structure with particles of kaolin. After 50 days of aging the suspension of the composite with concentration of 0.001% by silver had maintained its high antimicrobial activity, the concentration of NPS Ag in the supernatant was (0,054 0,011) g/g. The high antimicrobial activity of the obtained composite material allowed recommend it for application in medicine for the prevention and treatment of infections of different localization, as enter sorbent in the gastro-intestinal infections, dysbacteriosis, as well as in other areas of biology and economy.

KEYWORDS:
kaolin (white clay), "Kremnevit", silver nanoparticles, antimicrobial activity, physical properties
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