Home Page   -   Åditorial Board   -   Instructions to autors   -   Archive   -   Contact   -   Journal's Indexing UA   EN


Last issue

¹ 1 (110), 2024


Support

Edition of the journal is supported partially by the grant of the Fogarty International Center (FIC) of the National Institute of Health and Illinois University, Chicago.

JOURNAL

ISSN 2077-7477 (Print)
ISSN 2077-7485 (Online)

A journal "Dovkillia ta zdorovia" (Environment & Health) publishes the articles on the problems in the field of medical ecology, hygiene, health protection and ecological safety.

Founder of the Journal:
State Institution "O.M. Marzeiev Institute for Hygiene and Medical Ecology of the National Academy of Medical Sciences of Ukraine"

Frequency of publication:
quarterly

Environment & HealthISSN: 2077-7477 eISSN: 2077-7485
No: 3 (100)   -   September, 2021   -   Pages: 64-70
Substantiation of safe use of disinfectant «STERIZENT»
Surmasheva O.V.1, Babii V.F.1, Polka O.O.1, Chernysh O.O.1
1 State Institution "O.M. Marz³eiev Institute for Public Health of the NAMSU"

ÓÄÊ: 614.4

ABSTRACT:
diseases, especially a use of the disinfectants (DS), in particular, are of special relevance in order to break the mechanisms of the transmission of infectious agents. The variety of DS, presented at the modern market, allows us to choose the most appropriate ones for the purposes, conditions and methods of application. However, the effectiveness and safety of the DS both for humans and the environment are still the main choice criteria. In this regard, electrolysis preparations (anolytes) attract a special attention.

Objective: We determined the bactericidal, virucidal and yeast activity of the STERISENT preparation (Israel) according to modern requirements and standards.

Materials and methods: Studies of the specific activity of the DS "STERISENT" were carried out in accordance with the Order of the Ministry of Health of Ukraine No. 2024, 03.09.2020 and the European standards. The test cultures and culture media recommended by the Order No. 2024 and the standards EN 13727: 2012 + A2: 2015 [3] and DSTU EN 13624: 2019 (EN 13624: 2013, IDT) were used in the quantitative suspension method. Virucidal activity was studied in the intestinal bacteriophages T2 and MS2.Toxicological studies were carried out in the laboratory animals in accordance with modern standards.

Results: A high bactericidal and yeast-killing activity of STERISENT preparation (Israel) in undiluted form was established for Escherichia coli K12 NCTC 10538 lg R >5.42, for Pseudomonas aeruginosa lg R >5.37; for Staphylococcus aureus lg R >5.34; for Candida albicans lg R >4.31at exposure of 30 s and 60 s. In the study in bacteriophages at an exposure of 30 s, the logarithms of the reduction were: T2 - >5.08, MS2 - >5.12. According to GOST 12.1.007-76,by the parameters of acute toxicity, STERIZENT preparation belongs to class 4 - low-hazardous substances when administered to the stomach and applied to the skin; at the inhalation application in saturated concentrations - to class 3. moderately dangerous substances. The preparation does not have a sensitizing effect in the recommended modes of application.

Conclusions: The obtained results make it possible to recommend the STERISENT preparation (Israel) for wide practical use as a highly effective and one of the least toxic DS.

KEYWORDS:
disinfectants, bacteria, fungi, viruses
REFERENCES:
1. Pro zatverdzhennia Metodiv provedennia doslidzhen spetsyfichnoi aktyvnosti, bezpechnosti, yakosti (efektyvnosti) dezinfektsiinykh zasobiv ta yikh vyprobuvannia na praktytsi : Nakaz MOZ Ukrainy ¹ 03.09.2020 ¹ 2024 [On the Adoption of the Methods for the Performance of the Research of Specific Activity, Safety, Quality (Efficiency) of the Disinfectants and Their Practical Tests: Order of the Ministry of Health of Ukraine ¹ 03.09.2020 (2024]. (in Ukrainian). URL : https://moz.gov.ua/article/ministry-mandates/nakaz-moz-ukraini-vid-03092020--2024-pro-zatverdzhennja-metodiv-provedennja-doslidzhen-specifichnoi-aktivnosti-bezpechnosti-jakosti-efektivnosti-dezinfekcijnih-zasobiv-ta-ih-viprobuvannja-na-praktici
2. Chemical Disinfectants and Antiseptics – Preservation of Microbial Strains Used for the Determination of Bactericidal and Fungicidal Activity: EN 12353. URL : https://standards.iteh.ai/catalog/standards/cen/82ada8f0-65f8-4ee7-aa6e-946c12a1a673/en-12353-2021
3. Zasoby khimichni dezinfikuvalni ta antyseptyky. Kilkisnyi suspenziinyi metod otsiniuvannia dlia vyznachennia bakterytsydnoi aktyvnosti protylehionely khimichnykh dezinfikuvalnykh zasobiv dlia vodnykh system. Metod vyprobuvannia ta vymohy (etap 2, krok 1): DSTU EN 13623: 2019 (EN 13623:2010, IDT) [Chemical Disinfectants and Antiseptics. Quantitative Suspension Test for the Evaluation of Bactericidal Activity Against Legionella of Chemical Disinfectants for Aqueous Systems. Test Method and Requirements (Phase 2, Step 1) : DSTU EN 13623: 2019 (EN 13623:2010, IDT)]. (in Ukrainian). URL: https://zakon.rada.gov.ua/rada/show/v0176774-19#Text
4. Zasoby khimichni dezinfikuvalni ta antyseptyky. Kilkisnyi suspenziinyi metod otsiniuvannia dlia vyznachennia funhitsydnoi abo psevdoaktyvnosti v medychnii haluzi. Metod vyprobuvannia ta vymohy (etap 2, krok 1): DSTU EN 13624: 2019 (EN 13624:2013, IDT). [Chemical Disinfectants and Antiseptics. Quantitative Suspension Test for the Evaluation of Fungicidal or Yeasticidal Activity in the Medical Area. Test Method and Requirements (Phase 2, Step 1) : DSTU EN 13624: 2019 (EN 13624:2013, IDT)]. (in Ukrainian). URL: https://zakon.rada.gov.ua/rada/show/v0176774-19#Text
5. Khimichni dezinfikuvalni zasoby. Kilkisnyi suspenziinyi metod otsiniuvannia dlia vyznachennia virulitsydnoi aktyvnosti proty bakteriofahiv khimichnykh dezinfikuvalnykh zasobiv, vykorystovuvanykh u zakladakh kharchuvannia ta promyslovosti : DSTU EN 13610: 2019 (EN 13610:2002, IDT). [Chemical Disinfectants. Quantitative Suspension Test for the Evaluation of Virucidal Activity Against Bacteriophages of Chemical Disinfectants Used in Food and Industrial Areas. Test Method and Requirements (Phase 2, Step 1) : DSTU EN 13610: 2019 (EN 13610:2002, IDT)]. (in Ukrainian). URL: https://zakon.rada.gov.ua/rada/show/v0176774-19#Text
6. Sanitarno-mikrobiolohichnyi kontrol yakosti pytnoi vody : MV 10.2.1-113-2005 [Sanitary-and-Microbiological Control of Drinking Water Quality]. (in Ukrainian). URL : https://zakon.rada.gov.ua/rada/show/v0004488-10#Text
7. Derzhavni sanitarni pravyla i normy bezpeky produktsii parfumerno-kosmetychnoi promyslovosti: DSanPiN 2.2.9.027-99 [State Sanitary Rules and Safety Standards for the Products of Perfumery and Cosmetic Industry: SSanRS 2.2.9.027-99]. (in Ukrainian). URL: https://zakon.rada.gov.ua/rada/show/v0027588-99#Text
8. OECD Principles of Good Laboratory Practice: OECD Environmental Health and Safety Publications ENV/MC/CHEM(98)17. Paris; 1998 : 41ð.
9. Printsipy nadlezhashchey laboratornoy praktiki : GOST R 53434-2000 [Principles of Good Laboratory Practice : GOST R 53434-2000]. Moscow ; 2000 (in Russian).
10. Reznikov O.P. Zahalni etychni pryntsypy eksperymentiv na tvarynakh [General Ehic Principles of the Experiments in Animals]. Endokrynolohiia. 2001 ; 8 (1) : 142-145 (in Ukrainian).
Designed by: IPH NAMSU © Environment & Health, 2006-2024. All rights reserved.
Use of text and graphic materials of the site is allowed only upon written permission of the editoral board.